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A new method for isolation and differentiation of native Entamoeba histolytica and E. dispar cysts from fecal samples
B Walderich1, L Müller, R Bracha
1Institut für Tropenmedizin, Forschungslaboratorien, Tübingen, Germany. brigitte.walderich@uni-tuebingen.de
Parasitology Research
|January 1, 1997
Abstract:
A new method for the purification of protozoan cysts from feces was established, allowing to isolation of native cysts. The procedure consists of two sucrose-density gradients and enzymatic digestion of cellulose particles by cellulase and can be accomplished in a few hours. The cyst fractions were differentiated into Entamoeba histolytica and E. dispar using the DNA probes P145 and B133 and a dot-blot test.