Related Experiment Video

Updated: Aug 18, 2026

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
09:26

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Published on: March 26, 2017

Quantitative study of secondary structure of histones H1, H2A, and H4 in solution by infrared spectroscopy

B V Shestopalov, Y N Chirgadze

    European Journal of Biochemistry
    |August 1, 1976
    PubMed

    More Related Videos

    Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique06:32

    Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

    Published on: March 9, 2022

    Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry05:52

    Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry

    Published on: January 12, 2024

    Related Concept Videos

    2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

    2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

    Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
    Infrared (IR) Spectroscopy: Overview01:09

    Infrared (IR) Spectroscopy: Overview

    When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
    Different compounds display unique properties due to their...
    IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

    IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

    Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single stretching vibration...
    IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

    IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

    A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
    According to Hooke's law, the vibrational frequency is directly proportional to the...
    IR Frequency Region: X–H Stretching01:24

    IR Frequency Region: X–H Stretching

    In IR spectroscopy, signals produced by the X−H bonds (such as C−H, O−H, or N−H) can be observed in the frequency range of  2700–4000 cm–1. The C−H stretching vibration forms sharp bands in the region 2850–3000 cm–1. The presence of the O−H stretching vibration leads to the forming of an absorption band in the frequency range 3650–3200 cm−1. At the same time, N−H stretching can be confirmed by absorption bands in the 3500–3100 cm−1 range. Even though both O−H and N−H bonds vibrate at a similar...
    2D NMR: Overview of Heteronuclear Correlation Techniques01:18

    2D NMR: Overview of Heteronuclear Correlation Techniques

    Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.