Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Study of HIV localization in sperm]

N Bréchard1, P Galéa, F Silvy

  • 1Laboratoire d' Analyses de Biologie Médicale, Marseille.

Contraception, Fertilite, Sexualite (1992)
|May 1, 1997
PubMed
Summary

This study found no Human Immunodeficiency Virus (HIV) in mobile sperm cells. A screening procedure for HIV-free sperm enabled HIV-negative births in discordant couples, ensuring maternal and infant safety.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of monoclonal antibodies to pre-haptoglobin 2 and their use in an enzyme-linked immunosorbent assay (ELISA).

Journal of immunological methods·2014
Same author

Generation and molecular characterization of a monoclonal antibody reactive with conserved epitope in sphingomyelinases D from Loxosceles spider venoms.

Vaccine·2014
Same author

Cerebrospinal Aβ11-x and 17-x levels as indicators of mild cognitive impairment and patients' stratification in Alzheimer's disease.

Translational psychiatry·2013
Same author

The placenta contributes to activation of the renin angiotensin system in twin-twin transfusion syndrome.

Placenta·2008
Same author

Inflammatory cytokines in juvenile idiopathic arthritis: effects on physical growth and the insulin-like-growth factor axis.

Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society·2008
Same author

Stage I twin-twin transfusion syndrome: rates of progression and regression in relation to outcome.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2007

Area of Science:

  • Reproductive Biology
  • Virology
  • Molecular Biology

Context:

  • Human immunodeficiency virus (HIV) transmission risks during conception for serodiscordant couples.
  • Need for safe reproductive options for HIV-positive men and HIV-negative women.
  • Limitations of traditional methods like natural intercourse or washed sperm insemination.

Purpose:

  • To investigate the presence and localization of HIV in human ejaculate and its components.
  • To develop and validate a method for screening HIV-free spermatozoa.
  • To assess the safety and efficacy of using screened HIV-free sperm for assisted reproduction.

Summary:

  • Sixty-three ejaculates from 19 HIV-positive patients were analyzed using cellular and molecular methods.
  • HIV was not detected in living, mobile spermatozoa, but was found in seminal fluid and nuclear fractions.
  • A procedure for screening HIV-free sperm was successfully implemented, leading to 11 HIV-negative pregnancies in previously discordant couples.

Impact:

  • Established a safe and effective method for assisted reproduction in HIV-serodiscordant couples.
  • Prevented mother-to-child transmission of HIV through sperm screening.
  • Offers a secure alternative to natural intercourse, eliminating the 'Russian roulette' risk of transmission.

Related Experiment Videos