Related Experiment Video
Updated: Oct 23, 2026

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
Published on: June 27, 2015
Reevaluation of anionic sites in the rat glomerular basement membrane
1Department of Pathology, Chiba University School of Medicine, Chuo-Ku, Japan.
Abstract:
We reevaluated the distribution of anionic sites in the glomerular basement membrane with ruthenium red, alcian blue, cationic ferritin, polyethyleneimine, and cationic colloidal gold. Without the accentuation by the metal staining with uranyl acetate and lead citrate, we observed the existence of anionic sites throughout all the layers of the glomerular basement membrane with a similar reticular pattern and the structural difference between the lamina rara and lamina densa, indicating that the lamina rara is an actual structural element.
Related Concept Videos
Renal Corpuscle
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous capillaries...
Glomerular Filtration
Components of the Filtration Membrane
The filtration process involves three key layers: the glomerular endothelial cells, the basement membrane, and the podocyte-formed filtration slits.
Glomerular Filtration Rate and its Regulation
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
Renal Drug Excretion: Glomerular Filtration
Drugs gain access to the kidney via the renal artery, which progressively branches off into afferent arterioles.
Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate

