[The experimental treatment of laryngeal cancer using local draining lymph node lymphocytes]

In order to know whether laryngeal cancer draining lymph node lymphocytes (INL) have antitumor effects in vivo, we transfered IL-2 activated LNL to nude mice with human laryngeal squamous cell carcinoma line PHC3 xenografts, and observed a suppressive effect on tumor growth. It was also found in the epitype changes of LNL by indirect immunofluorencence method the major composition of freshly isolated LNL was CD3+ T cells in which there were more Th/i cells than Ts/c cells, CD4+/CD8+ ratio was 3.30 +/- 0.653. No evidence of lymphocytes multiplication or CD3+, CD4+, CD8+ epitype changes was found after short term IL-2 culture, but we found more HLA-DR+, CD25+ cells which means there was an increase in activated lymphocytes after culture. The experiment suggests that laryngeal cancer draining lymph node lymphocytes have antitumor effects in vivo after IL-2 short term culture, and can be used in clinical immunotherapy.