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[Expression of IL-8 mRNA in glomerular endothelial cells]
Zhonghua Yi Xue Za Zhi
|June 1, 1996
Abstract
Objective:
To study the expression of IL-8 mRNA in cultured human glomerular endothelial cells.
Method:
The effects of IL-1 beta and TNF alpha on the production of IL-8 by glomerular endothelial cells were also observed. RT-PCR was used.
Results:
There was only weak expression of IL-8 mRNA in cultured human glomerular endothelial cells without the presence of any stimulating factors, whereas the expression of IL-8 mRNA in cultured human glomerular endothelial cells was significantly increased after the glomerular endothelial cells have been treated with IL-1 beta (25 u/ml) or TNF alpha (10 ng/ml) for 24 hours.
Conclusion:
Glomerular endothelial cell injury may enhance the expression of IL-8, which mediates the inflammatory reactions in glomeruli.