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[The protective effects of dichloroacetate on cerebral ischemia after reperfusion of fed rats]
1Department of Neurology, Second Affiliated Hospital, Hubei Medical University, Wuhan.
Objective:
To study the brain protective mechanisms of dichloroacetate (DCA) by observing the influence of DCA on the biochemical and pathological changes in ischemic brain tissues in different periods of reperfusion.
Methods:
The FED-RAT cerebral ischemic model induced by 4-vessel occlusion was applied. 55 mature male Sprague-Dawley rats were divided into the control group, normal saline and DCA-treated groups before ischemia, normal saline and DCA-treated groups after ischemia equally and randomly.
Results:
DCA could significantly lower the brain lactic acid, water content, and the diameter of cortical neurons, and protect the pathological damage of the membranaceous structure, before or after ischemia at a dose of 25 mg/kg, compared with the normal saline treated groups.
Conclusion:
Lowering brain lactate, resisting brain edema and protecting the membranaceous structures are the main brain protective mechanisms of DCA in biochemistry and ultrastructure.