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Enhanced nucleolar parameters in Vicia faba L. induced by 5-azacytidine may be only partly attributed to DNA
M J Olszewska1, T Sakowicz, J Kaźmierczak
1Department of Plant Cytology and Cytochemistry, University of Lódź, Poland.
Abstract:
We aimed to find if diminishing rDNA expression in differentiating and differentiated root parenchyma, in spite of DNA endoreplication, could be related to rDNA methylation. The effect of 5-azacytidine (10(-6)M, 72 h) was studied in successive segments of Vicia faba roots. In 5-azacytidine-treated cells, nucleolar parameters: [3H] uridine incorporation, size, amount of AgNOR protein and the number of bi-nucleolate cells were increased. [3H] uridine incorporation and the amount of AgNOR protein were significantly correlated. In meristems, HPLC analysis demonstrated a decrease in m5dC content by 3.7%. Southern hybridization showed demethylation at CCGG sites in repetitive BamHI sequences and discrete demethylation in coding region of rDNA.