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Related Experiment Videos

Computer-assisted sperm analysis for assessing initial semen quality and changes during storage at 5 degrees C

A L Tardif1, P B Farrell, V Trouern-Trend

  • 1Department of Animal Science, Cornell University, Ithaca, NY 14853, USA.

Journal of Dairy Science
|August 1, 1997
PubMed
Summary

Computer-assisted sperm analysis accurately assessed bull sperm motility and characteristics across different extenders. This technology effectively detects variations among bulls and sperm changes during storage.

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Area of Science:

  • Veterinary Science
  • Reproductive Biology
  • Animal Breeding

Background:

  • Sperm quality is crucial for artificial insemination success in cattle.
  • Accurate and efficient methods are needed to evaluate bull sperm characteristics.
  • Cryopreservation and extenders significantly impact sperm viability.

Purpose of the Study:

  • To evaluate bull sperm characteristics using computer-assisted sperm analysis (CASA).
  • To compare sperm parameters in different semen extenders (modified Tyrode's, Cornell University, and egg yolk-glycerol-Tris).
  • To assess the impact of cooling and storage on bull sperm quality.

Main Methods:

  • Semen from eight bulls was analyzed using the Hamilton Thorne integrated visual optical system.
  • Sperm were evaluated in modified Tyrode's solution, Cornell University extender, and egg yolk-glycerol-Tris extender.

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  • Videotaping of sperm in 20-micron chambers allowed analysis of eight sperm characteristics.

    • Main Results:

    • Significant differences in sperm characteristics were observed among bulls.
    • Initial motility was highest in modified Tyrode's solution (87%), followed by Cornell University extender (79%) and egg yolk-glycerol-Tris extender (66%).
    • Sperm velocity slightly declined during 3 days of storage, while hyperactive sperm increased.

    Conclusions:

    • Computer-assisted sperm analysis provides rapid and accurate measurement of bull sperm characteristics.
    • The study demonstrated the utility of CASA in detecting inter-bull variability in sperm quality.
    • Egg yolk-glycerol-Tris extender maintained sperm quality adequately during short-term storage.