Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Discriminating between barley genotypes using microsatellite markers

J Russell1, J Fuller, G Young

  • 1Scottish Crop Research Institute, Invergowrie, Dundee, Scotland, U.K.

Genome
|August 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reducing Variation in Palliative Treatment for Oesophago-Gastric Cancer: Insights from the National Oesophago-Gastric Cancer Audit.

Clinical oncology (Royal College of Radiologists (Great Britain))·2026
Same author

Assessing the vulnerability of commercial bivalves to intensifying atmospheric heatwaves in coastal ecosystems.

Marine environmental research·2025
Same author

The effect of morphine in the development of neonatal cholestasis in extremely preterm neonates.

Journal of neonatal-perinatal medicine·2025
Same author

Nuclear Astrophysics in the Storage Ring: Background Suppressed Simultaneous Measurement of (p,γ) and (p,n) Reactions.

Physical review letters·2025
Same author

First Measurement of the Neutron-Emission Probability with a Surrogate Reaction in Inverse Kinematics at a Heavy-Ion Storage Ring.

Physical review letters·2025
Same author

A new approach for assessing the radioecological risk associated with the legacy discharge of oil derived natural radioactivity in the UK North Sea.

Marine pollution bulletin·2025
Same journal

Two satellite repeats reveal B chromosome structural diversity in Atractylodes lancea.

Genome·2026
Same journal

A Drosophila teissieri I-element retrotransposon's ORF1p shows RNA binding cis-preference in the D. melanogaster female germline.

Genome·2026
Same journal

Abiotic stress-responsive tRNA-derived fragments in pitanga (<i>Eugenia uniflora</i> L.): regulatory roles in drought and salinity adaptation.

Genome·2026
Same journal

Diacylglycerol kinase promotes forgetting of aversive olfactory memory in Drosophila.

Genome·2026
Same journal

From lab bench to public voice: how to incorporate science communication into your research program.

Genome·2026
Same journal

<i>delimtools</i>: an R package for species delimitation.

Genome·2026
See all related articles

Microsatellite markers effectively identified 24 barley genotypes, even those with similar pedigrees. This genetic analysis also corrected a long-standing error in the pedigree of the Golden Promise barley cultivar.

Area of Science:

  • Agricultural Science
  • Genetics
  • Plant Breeding

Background:

  • Accurate identification and differentiation of plant genotypes are crucial for breeding programs and cultivar registration.
  • Traditional methods like botanical descriptors may not always provide sufficient discriminatory power for closely related genotypes.

Purpose of the Study:

  • To evaluate the efficacy of microsatellite loci for distinguishing between barley genotypes.
  • To assess the stability and reliability of microsatellites in genetic analyses across generations.
  • To resolve ambiguities in existing barley cultivar pedigrees.

Main Methods:

  • Genotyping of 24 barley genotypes (23 cultivars, 1 breeding line) using eleven microsatellite loci.
  • Analysis of three distinct combinations of four microsatellites to determine their discriminatory power.

Related Experiment Videos

  • Retrospective analysis of the Golden Promise barley pedigree using microsatellite data.

    • Main Results:

    • Three combinations of four microsatellite loci achieved probabilities of identity < 1 in 1000, distinguishing all 24 genotypes.
    • Microsatellites differentiated genotypes with identical pedigrees, outperforming botanical descriptors.
    • Stable microsatellite patterns were observed across generations, confirming their reliability.

    Conclusions:

    • Microsatellite markers provide a robust and highly discriminatory tool for barley genotype identification and pedigree analysis.
    • The study corrected a historical inaccuracy in the pedigree of Golden Promise, identifying its ancestor as Irish Goldthorpe.
    • Microsatellite analysis offers a powerful complement to traditional methods in plant breeding and genetic research.