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Isolation of Vibrio cholerae 0139 phages to develop a phage typing scheme
A K Chakrabarti1, A N Ghosh, B L Sarkar
1Vibrio Phage Reference Laboratory, National Institute of Cholera & Enteric Diseases, Calcutta.
Abstract:
Five V. cholerae 0139 phages isolated from different parts of India have been used for phage typing study. A strain isolated from Nagpur city (NPR-4) was used as the host for phage propagation. All but one of the 260 strains of V. cholerae 0139 were found to be typeable and could be clustered into 8 distinct phage types as revealed by lytic patterns. Phage type 1 was the predominant type (61.15%) followed by type 2 (18.46%). The strains isolated from Madras exhibited 7 out of 8 phage types. These newly isolated phages could be adopted for phage typing of V. cholerae 0139 strains as an epidemiological tool.