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Analysis of phospholipid molecular species
A Vecchini1, V Panagia, L Binaglia
1Department of Biochemistry, University of Perugia, Italy.
Molecular and Cellular Biochemistry
|July 1, 1997
Abstract:
A method is described for analysing molecular species of glycerophospholipids. Diglycerides obtained by phospholipase C-catalysed hydrolysis of the phospholipid are separated into the diacyl- alkylacyl- and alkenylacyl- subclasses by HPLC on silicic acid. The molecular species of diacylglycerol are separated by HPLC of underivatised diglycerides on a reverse phase octadecyl-silica column.