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Related Experiment Videos

Cell coupling and uncoupling in the ventricular zone of developing neocortex

K Bittman1, D F Owens, A R Kriegstein

  • 1Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269-4156, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|September 15, 1997
PubMed
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Neural precursor cells in the developing neocortex form clusters via gap junctions. This clustering is dynamic and may regulate neurogenesis by influencing cell cycle progression.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Cells in the developing neocortex's ventricular zone (VZ) form clusters through gap junction channels.
  • The function of this clustering in cortical neurogenesis remains unclear.
  • Clustering allows for localized interactions among precursor cells and other VZ cells.

Purpose of the Study:

  • To investigate the cell types involved in VZ clustering.
  • To determine the cell cycle timing of VZ cell coupling and uncoupling.
  • To understand the role of clustering in regulating neurogenesis.

Main Methods:

  • 5-bromo-2'-deoxyuridine (BrDU) pulse labeling
  • Intracellular biocytin labeling
  • Immunocytochemistry

Related Experiment Videos

  • Pharmacological uncoupling with octanol

    • Main Results:

    • VZ clusters comprise radial glia and neural precursors, excluding differentiating or migrating neurons.
    • In early neurogenesis, most cells in S and G2 phases are coupled, along with half of G1 cells.
    • In late neurogenesis, G2 cells remain coupled, but a significant portion of G1 and S phase cells become uncoupled.
    • Pharmacological uncoupling reduced the percentage of VZ cells entering the S phase.

    Conclusions:

    • Cell clustering in the VZ is specific to neural precursors and radial glia.
    • The coupling of VZ cells is dynamic throughout the cell cycle and changes during neurogenesis.
    • VZ cell clustering plays a role in regulating the cell cycle and neurogenesis.