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Pressure-induced apoptosis in human lymphoblasts

K J Takano1, T Takano, Y Yamanouchi

  • 1Institute of Materials Science, University of Tsukuba, Tsukuba, Japan. takano@mat.ims.tsukuba.ac.jp

Experimental Cell Research
|August 25, 1997
PubMed
Summary

High pressure exposure causes human lymphoblast cell death through necrosis and apoptosis. Cell cycle phase, particularly the S phase, influences the rate of pressure-induced cell death.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Biophysics

Background:

  • Cellular response to mechanical stress is crucial in various physiological and pathological processes.
  • Understanding the impact of high hydrostatic pressure (HHP) on human cells is vital for applications in food processing, medicine, and research.
  • Human lymphoblasts are a relevant model for studying cellular responses due to their rapid proliferation.

Purpose of the Study:

  • To investigate the viability of human lymphoblasts following exposure to high hydrostatic pressure (HHP).
  • To determine the mechanisms of cell death (necrosis and apoptosis) induced by HHP.
  • To explore the influence of the cell cycle on pressure-induced cell death.

Main Methods:

  • Human lymphoblasts were subjected to compression at 37°C for 30 minutes at pressures ranging from 85 to 200 MPa.

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  • Cell viability was assessed immediately after decompression.
  • Apoptosis was detected using the Tunel method, optical microscopy, and electron microscopy.
  • Cell cycle phase was analyzed to correlate with cell death.

    • Main Results:

    • Immediate post-decompression revealed necrotic cells.
    • Cell viability decreased sigmoidally from 85 to 200 MPa.
    • Apoptotic cells were observed 8 hours after decompression at 100 MPa.
    • Apoptosis was confirmed as pressure-induced.
    • Both necrosis and apoptosis were accelerated in the S phase of the cell cycle.

    Conclusions:

    • High hydrostatic pressure induces both necrosis and apoptosis in human lymphoblasts.
    • The S phase of the cell cycle is particularly sensitive to pressure-induced cell death.
    • These findings have implications for understanding cellular responses to mechanical stress and HHP applications.