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Related Experiment Videos

Changes in actin filament organization during pseudopod formation

E Lee1, E A Shelden, D A Knecht

  • 1Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269, USA.

Experimental Cell Research
|August 25, 1997
PubMed
Summary

New research shows that during cell movement, the actin cortex beneath new pseudopods breaks down. This suggests cells build new structures rather than reusing existing actin networks.

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Area of Science:

  • Cell biology
  • Biochemistry
  • Cytoskeleton dynamics

Background:

  • Actin dynamics are crucial for cell motility.
  • Understanding pseudopod formation is key to cell migration.

Purpose of the Study:

  • To visualize dynamic changes in F-actin localization during pseudopod extension in Dictyostelium amoebae.
  • To investigate the relationship between existing actin cortex and new pseudopod formation.

Main Methods:

  • Introduction of fluorescent phalloidin into Dictyostelium amoebae.
  • Microscopic observation of F-actin localization during pseudopod extension.
  • Comparison of wild-type cells and myosin II heavy chain-lacking cells.

Main Results:

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  • Fluorescent phalloidin initially localized to the cell's peripheral cortex.
  • Newly formed pseudopods lacked fluorescence, indicating phalloidin binds to existing F-actin.
  • The fluorescent signal disappeared from the region underlying the expansion zone as pseudopod extension proceeded.
  • Similar results were observed in both wild-type and myosin II heavy chain-lacking cells.

    • Conclusions:

    • New pseudopods are not constructed upon the pre-existing actin cortex.
    • The actin cortex is locally disassembled during the formation of new actin networks for pseudopod extension.