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Updated: Aug 16, 2026

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
Published on: February 3, 2020
A nuclear polyhedrosis virus from the obliquebanded leafroller, Choristoneura rosaceana (Harris)
1Canadian Forest Service-Atlantic Forestry Centre, Natural Resources Canada. Fredericton, New Brunswick, Canada. clucarot@nrcan.gc.ca
Abstract:
A nuclear polyhedrosis virus (CrMNPV) has been isolated from larvae of the obliquebanded leafroller Choristoneura rosaceana (Harris) collected near St. Quentin in northwest New Brunswick, Canada. Restriction endonuclease digestion-gel electrophoresis of this virus, using a number of different endonucleases and whole CrMNPV genome Southern hybridization, have shown it to be different but similar to the equivalent virus from the spruce budworm Choristoneura fumiferana (Clem.) (CfMNPV). The polyhedrin gene of CrMNPV has been isolated, cloned, and sequenced and shown to have a predicted amino acid sequence only two amino acids different from the CfMNPV polyhedrin. In bioassays against spruce budworm larvae, CrMNPV had little pathogenicity to that host.
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