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Updated: Jul 18, 2026

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
Published on: June 20, 2010
Binding of nascent glucuronoxylan to the cell walls of pea seedlings
C T Brett1, S A Healy, M S McDonald
1Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, UK. c.brett@bio.gla.ac.uk
Abstract:
Glucuronoxylan synthesised in vitro by membrane-bound enzymes from etiolated pea epicotyls was found to bind to isolated cell walls from the same tissue in a pH-dependant manner. The binding was maximum at pH 3.5-4.0, and decreased to zero at pH 6. The bound glucuronoxylan could be dissociated from the cell walls by washing at pH 6, and the binding appeared to be non-covalent. Extraction experiments indicated that the glucuronoxylan was binding to hemicellulose in the cell-wall. The observed binding may be significant in the process of cell-wall assembly in vivo.
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