Insulin responses to a fat meal in hypothalamic microdialysates and plasma

In a recent microdialysis study in freely-behaving rats, we observed changes in immunoreactive insulin (IRI) in hypothalamic dialysates after a meal of standard laboratory chow. These changes did not always parallel plasma insulin variations, suggesting a partial independence from peripheral insulin. In the present study, we have attempted to assess the profile of medial hypothalamus (VMPH-PVN) extracellular insulin and peripheral insulin before and after a fat meal (lard). In contrast to the increase we previously observed with chow meals, hypothalamic extracellular IRI decreased during the fat meal and fell to 60% 30 min after the meal. Plasma insulin levels did not change. The intake of the lard meal, provided in unlimited amounts, was much larger in calories than the intake of a chow meal under the same conditions. However, when rats were offered a meal of chow after they had eaten a meal averaging 6.7 g of fat (61 calories), they immediately began eating the chow. Thus, the meal of fat produced no general satiation. On the contrary, the rats consumed a second chow meal only after a delay of approximately 40 min after the first one. The present data, in conjunction with our previous observations with chow fed rats, suggest that the level of extracellular hypothalamic IRI may decrease independently of plasma insulin levels and may, at least partially, account for the observed lack of satiation.