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Related Experiment Videos

SAGE transcript profiles for p53-dependent growth regulation

S L Madden1, E A Galella, J Zhu

  • 1Department of Molecular & Cellular Biology, PharmaGenics, Inc., Allendale, NJ 07401, USA.

Oncogene
|August 28, 1997
PubMed
Summary
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Serial analysis of gene expression (SAGE) identified 14 genes regulated by functional p53 protein in rat cells. This study highlights SAGE

Area of Science:

  • Molecular Biology
  • Genomics
  • Cancer Research

Background:

  • The p53 tumor suppressor protein plays a critical role in cellular responses to stress.
  • Understanding p53's transcriptional targets is crucial for comprehending its tumor suppressor functions.
  • Serial analysis of gene expression (SAGE) provides a comprehensive method for gene expression profiling.

Purpose of the Study:

  • To identify genes whose expression is dependent on functional p53 protein using SAGE.
  • To compare gene expression profiles in cells with functional versus non-functional p53.
  • To investigate the impact of temperature-sensitive p53 mutations on global gene expression.

Main Methods:

  • Utilized Serial analysis of gene expression (SAGE) technology.

Related Experiment Videos

  • Analyzed gene expression profiles in rat embryo fibroblast cells with temperature-sensitive p53.
  • Compared gene expression at permissive and non-permissive temperatures for p53 function.

    • Main Results:

    • Identified 14 genes (P < 0.001) whose expression was dependent on functional p53.
    • Observed increased expression of RAS, U6 snRNA, cyclin G, and EGR-1 with functional p53.
    • Found elevated expression of actin, tubulin, and HSP70 at non-permissive temperatures.

    Conclusions:

    • Demonstrated that functional p53 regulates the expression of specific genes.
    • Highlighted the utility of SAGE for evaluating global transcriptional responses.
    • Suggested that different p53 mutant proteins may lead to distinct transcriptional profiles.