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Related Experiment Videos

Protein-protein interactions in the synaptonemal complex

M Tarsounas1, R E Pearlman, P J Gasser

  • 1Department of Biology, York University, Toronto, Ontario, Canada.

Molecular Biology of the Cell
|August 1, 1997
PubMed
Summary

Mammalian chromosome core proteins Cor1 and Syn1 interact homotypically and with Ubc9. These interactions may be crucial for synaptonemal complex regulation during meiosis.

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Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • Cor1 (M(r) 30,000) is a major component of meiotic prophase chromosome cores.
  • Syn1 (M(r) 125,000) is found in synapsed homologous cores, forming the synaptonemal complex (SC).
  • Immunolocalization suggests potential homo- and heterotypic interactions between Cor1 and Syn1.

Purpose of the Study:

  • To investigate protein-protein associations between Cor1 and Syn1 using a yeast two-hybrid system.
  • To identify regions involved in Cor1 homotypic interactions.
  • To explore interactions between Cor1, Syn1, and Ubc9.

Main Methods:

  • Yeast two-hybrid system in Saccharomyces cerevisiae.
  • Testing various combinations of hamster Cor1 and Syn1 protein segments.

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  • In vitro affinity coprecipitation experiments.
  • Testing interactions with human and hamster Ubc9 homologues.

    • Main Results:

    • Cor1 exhibits homotypic interactions involving coiled-coil forming regions.
    • No interactions were detected between Cor1 and central/C-terminal Syn1 fragments.
    • Both Cor1 and Syn1 associate with Ubc9 (human Hsubc9 and hamster homologue).

    Conclusions:

    • Cor1 homotypic interactions are mediated by coiled-coil regions.
    • Syn1 fragments tested do not interact with Cor1 or other Syn1 fragments.
    • Interactions with Ubc9 may play a role in SC disassembly and sister centromere cohesiveness regulation during meiosis.