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Angiotensinogen mRNA is synthesized locally in rat ocular tissues
M Murata1, M Nakagawa, S Takahashi
1Department of Ophthalmology, Yamagata University School of Medicine, Japan.
Abstract:
Angiotensinogen (ANG) is present in the eye. However, the source has not been determined. We showed that ANG mRNA was present in several ocular tissues using RT-PCR with ANG-specific primers. One of 10 iris samples (10%), 1 of 10 ciliary body samples (10%), 3 of 10 choroid samples (30%) and 9 of 10 retina samples (90%) were positive for ANG. Our study provides the first direct evidence that the renin-angiotensin system (RAS) component ANG is synthesized locally in the eye, especially the retina and choroid portion. These are strategic locations for an ocular RAS to influence chorioretinal blood flow, raising the possibility that RAS alterations may be involved in chorioretinal vascular disease.
Insights
Scientists found angiotensinogen (ANG) is produced in the eye, particularly the retina and choroid. This suggests a local ocular renin-angiotensin system (RAS) may impact eye blood flow and vascular disease.
Area of Science:
- Ophthalmology
- Cardiovascular Biology
- Molecular Biology
Background:
- Angiotensinogen (ANG) is a key component of the renin-angiotensin system (RAS).
- While ANG is known to be present in the eye, its ocular source has remained undetermined.
- The potential role of an intraocular RAS in ocular health and disease is largely unexplored.
Purpose of the Study:
- To investigate the local synthesis of angiotensinogen (ANG) within various ocular tissues.
- To determine if the retina and choroid are sites of ANG production.
- To explore the implications of local ANG synthesis for ocular vascular regulation.
Main Methods:
- Reverse transcription polymerase chain reaction (RT-PCR) was employed to detect ANG mRNA.
- ANG-specific primers were utilized for sensitive and accurate detection.
- Ocular tissues including iris, ciliary body, choroid, and retina were analyzed.
Main Results:
- ANG mRNA was detected in 10% of iris samples and 10% of ciliary body samples.
- A higher prevalence of ANG mRNA was observed in 30% of choroid samples.
- The retina showed the highest incidence, with 90% of samples testing positive for ANG mRNA.
Conclusions:
- This study provides the first direct evidence for local synthesis of angiotensinogen (ANG) within the eye.
- The retina and choroid are identified as significant sites of ocular ANG production.
- The presence of a local ocular RAS, particularly in the retina and choroid, suggests a potential role in regulating chorioretinal blood flow and may be implicated in vascular diseases.