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Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window
Published on: November 19, 2010
A chronic model to simultaneously measure intracranial pressure, cerebral blood flow, and study the pial
J Jallo1, R Saetzler, C Mishke
1Temple University Health Sciences Center, Department of Neurosurgery, Temple University Hospital, Philadelphia, PA 19140, USA.
Abstract:
In an effort to study changes in cerebral blood flow (CBF), intracranial pressure (ICP) and intracranial compliance (ICC) simultaneously, we have developed a chronic model in rats using a pial window crown with two ports. This model can also be used to study vasoreactivity of pial vessels. Female Sprague-Dawley rats weighing between 225-250 g underwent placement of cranial chamber with dual ports under pentobarbital anesthesia. To test the utility of this technique 45 groups of rats were studied. Group 1 consisted of control animals. Group 2 consisted of rats undergoing 15 min of global cerebral ischemia. Rats in group 3 were evaluated for changes in vessel diameter and ICP after adenosine injection. In group 4 leukocyte/endothelial interactions were evaluated. These groups demonstrate the ability of this model to monitor CBF, ICP, ICC and pial vessel architecture in chronic rat experiments.
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