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Related Experiment Videos

Cloning heterologous genes: problems and approaches

J Agnan1, C Korch, C Selitrennikoff

  • 1Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, Denver 80262, USA. agnanj@essex.uchsc.edu

Fungal Genetics and Biology : FG & B
|June 1, 1997
PubMed
Summary

Cloning genes from pathogenic fungi is challenging. This review outlines three key strategies—functional complementation, sequence similarity, and genetic linkage—to facilitate the study of fungal pathogens.

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Area of Science:

  • Mycology
  • Molecular Biology
  • Genetics

Background:

  • Model fungi like *Neurospora crassa*, *Aspergillus niger*, and *Saccharomyces cerevisiae* are well-characterized genetically.
  • Many agriculturally and medically important pathogenic fungi remain poorly understood genetically.
  • This knowledge gap hinders research into fungal pathogenesis and the development of control strategies.

Purpose of the Study:

  • To propose and review strategies for cloning genes from pathogenic fungi using information from model organisms.
  • To address the challenges associated with characterizing genes in less-studied fungal pathogens.

Main Methods:

  • Functional complementation: Utilizing gene function to identify and isolate homologous genes.
  • Sequence similarity: Employing known sequences from model fungi to find related genes in pathogens.

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  • Genetic linkage: Analyzing the co-inheritance of genes to map and identify target sequences.
  • Key techniques include Southern hybridization, library screening, and the polymerase chain reaction (PCR).

    • Main Results:

    • Outlines three fundamental strategies for cloning cognate genes from pathogenic fungi.
    • Details the application of molecular techniques such as Southern hybridization and PCR.
    • Identifies common problems encountered during gene cloning in pathogenic fungi.

    Conclusions:

    • Gene sequences from model fungi are invaluable resources for studying pathogenic fungi.
    • The proposed strategies offer viable approaches to overcome challenges in cloning fungal pathogen genes.
    • Provides solutions to facilitate genetic analysis and enhance understanding of fungal pathogens.