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Related Experiment Videos

Plasminogen in proliferative vitreoretinal disorders

P Esser1, K Heimann, K U Bartz-Schmidt

  • 1Department of Vitreoretinal Surgery, University Eye Clinic Cologne, Germany.

The British Journal of Ophthalmology
|July 1, 1997
PubMed
Summary
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Intravitreal plasminogen is elevated in proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR), suggesting it may contribute to traction membrane formation. This finding indicates abundant substrate for fibrinolysis after vitrectomy.

Area of Science:

  • Ophthalmology
  • Vitreoretinal Surgery
  • Biochemistry

Background:

  • Intravitreal fibrin formation is common after vitrectomy for PVR, PDR, and endophthalmitis.
  • Plasminogen activators (PA) are used to manage this complication, requiring plasminogen substrate in the vitreous.

Purpose of the Study:

  • To quantify plasminogen levels in the vitreous of patients undergoing vitrectomy.
  • To investigate the role of plasminogen in postoperative fibrin formation and traction membrane development.

Main Methods:

  • Plasminogen was quantified in vitreous samples from 60 patients (PVR, PDR, macular pucker) using streptokinase activation and a chromogenic substrate.
  • Immunoblot analysis and immunocytochemistry confirmed plasminogen presence in vitreal proteins and epiretinal membranes.

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Main Results:

  • Plasminogen levels were significantly higher in PVR and PDR patients compared to controls.
  • Plasminogen localized to the extracellular matrix of epiretinal membranes.
  • Perivascular staining in PDR suggests blood-retinal barrier breakdown as a source of intravitreal plasminogen.

Conclusions:

  • Plasminogen may contribute to traction membrane formation in PVR and PDR.
  • Presurgical vitreous contains ample plasminogen, serving as a substrate for PA-mediated fibrinolysis post-vitrectomy.