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Serum isoamylase pattern in obstructive pancreatic disease
Scandinavian Journal of Gastroenterology
|January 1, 1977
Summary
Pancreatic serum isoamylases, separated by electrophoresis, show altered anodal fractions in duct obstruction. This finding aids in diagnosing pancreatic conditions like stenosis and occlusion.
Area of Science:
- Biochemistry
- Medical Diagnostics
- Gastroenterology
Background:
- Serum isoamylases are crucial biomarkers for pancreatic function.
- Electrophoresis separates pancreatic amylase into distinct fractions.
- Altered isoamylase patterns can indicate pancreatic disease.
Purpose of the Study:
- To investigate the behavior of pancreatic serum isoamylases in pancreatic duct obstruction.
- To determine if specific isoamylase fractions can serve as indicators for pancreatic main duct issues.
Main Methods:
- Agarose gel electrophoresis was used to separate pancreatic serum isoamylases.
- Isoamylase activity was quantified and compared between fractions.
- Analysis was performed on normal subjects and patients with pancreatic duct conditions.
Main Results:
- In normal subjects and acute hyperamylasemia, anodal pancreatic amylase is a minor fraction (<20%).
- Pancreatic duct occlusion or stenosis leads to a disproportionate increase in the anodal isoamylase fraction.
- The anodal fraction can exceed the main fraction's activity in obstructive cases, often with elevated total amylase.
Conclusions:
- The anodal fraction of pancreatic serum amylase is a sensitive indicator of pancreatic main duct obstruction or stenosis.
- Electrophoretic analysis of isoamylase provides valuable diagnostic information for pancreatic diseases.