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Hydrocortisone 17-butyrate degradation in the presence of micro-organisms
S M Rabouan-Guyon1, C M Fauvaud, P Y Courtois
1Faculty of Pharmacy, Poitiers University Hospital, France.
Objective:
This study compared the degradation of hydrocortisone 17-butyrate (H17B) in the presence of six different bacteria, commonly found on psoriatic skin.
Method:
H17B and its degradation products (hydrocortisone and hydrocortisone 21-butyrate (H21B)) were assayed by HPLC.
Results:
In the absence of micro-organisms, we observed 16.6 +/- 7.1% degradation. In the presence of micro-organisms and otherwise similar conditions, we noted that H17B degradation was not modified by cocci (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae). Three bacilli increased degradation, Escherichia coli 59.1 +/- 19.4%, Klebsiella oxytoca 62.1 +/- 6.7% and Pseudomonas aeruginosa 56.0 +/- 17.9%.
Conclusion:
The degradation of H17B into hydrortisone and H21B may produce a loss of therapeutic activity.