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Cloning and sequence of a thermostable multidomain xylanase from the bacterium Rhodothermus marinus
E Nordberg Karlsson1, E Bartonek-Roxå, O Holst
1Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Sweden. eva.nordberg-karlsson@biotek.lu.se
Biochimica Et Biophysica Acta
|August 7, 1997
Abstract:
The gene (xyn1) encoding a Rhodothermus marinus xylanase has been cloned and expressed in Escherichia coli. The gene comprises 5 different domains in an unusual combination. The cellulose binding domains (CBDs) encoded by xyn1 are repeated in tandem at the N-terminus and show similarity with the CBD family IV. The xyn1-gene is the first example encoding a CBD family IV in combination with a xylan hydrolyzing catalytic domain of the glycosyl hydrolase family 10.