The lipid bilayer determines helical tilt angle and function in lactose permease of Escherichia coli

The structure of lactose permease from Escherichia coli in its lipid environment was studied by attenuated total reflection Fourier transform infrared spectroscopy. The protein exhibits an alpha-helical content of about 65% and about 25% beta-sheet. Unusually fast hydrogen/deuterium (H/D) exchange to 90-95% completion suggests a structure that is highly accessible to the aqueous phase. An average tilt angle of 33 degrees for the helices was found with respect to the bilayer normal at a lipid-to-protein ratio of approximately 800:1 (mol/mol), and the permease exhibits optimal activity under these conditions. However, upon decreasing the lipid-to-protein ratio, activity decreases continuously in a manner that correlates with the decrease in the lipid order parameter and the increase in the average helical tilt angle. Taken together, the data indicate that the structure and function of the permease are strongly dependent on the order and integrity of the lipid bilayer.