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Decrease and recovery in cell surface charge induced by anesthetics

K Sato, K Kojima, C Sato

    The Tohoku Journal of Experimental Medicine
    |October 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

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    Intravenous anesthetics like thiopental and droperidol reduce red blood cell mobility. Recovery is possible within 2 hours, but high concentrations can cause irreversible effects on erythrocyte membranes.

    Area of Science:

    • Biophysics
    • Pharmacology
    • Cell Biology

    Background:

    • Anesthetics can alter cell membrane properties.
    • Understanding anesthetic effects on cell membranes is crucial for patient safety.
    • Erythrocyte electrophoretic mobility is a sensitive indicator of membrane changes.

    Purpose of the Study:

    • To investigate the impact of intravenous anesthetics on red blood cell membranes.
    • To determine the concentration-dependent effects of thiopental and droperidol.
    • To assess the reversibility of anesthetic-induced membrane alterations.

    Main Methods:

    • Cell electrophoresis was used to measure rat erythrocyte mobility.
    • Cells were incubated with varying concentrations of thiopental and droperidol.

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  • Recovery of mobility was assessed after anesthetic washout.

    • Main Results:

    • Anesthetic incubation decreased erythrocyte electrophoretic mobility by up to 25% within 30 minutes.
    • Thiopental showed a gradual effect (0.5-1000 µg/ml), while droperidol had a marked effect (0.3-1 µg/ml).
    • Complete mobility recovery occurred at 5 µg/ml thiopental or 1 µg/ml droperidol; higher concentrations led to irreversible changes, with a critical reversibility time of approximately 2 hours.

    Conclusions:

    • Intravenous anesthetics alter red blood cell membrane properties, affecting cell mobility.
    • The effects are concentration-dependent and exhibit a time-limited reversibility.
    • Cell electrophoresis is a valuable tool for evaluating anesthetic-membrane interactions.