Related Experiment Videos
[Cultures of corneal epithelium on hydrogel for synthetic epikeratoplasty]
T Bourcier1, V Borderie, S Carvajal-Gonzalez
1Service d'Ophtalmologie, Hôpital Saint-Antoine, Paris.
Purpose:
To improve the LLH480 hydrogel cytocompatibility and to assess the interactions between corneal epithelium and biomaterials.
Methods:
Rabbit corneal epithelium organotypic cultures were carried out on hydrogel samples coated with fibronectin, laminin, type IV collagen, or heparan sulfate proteoglycan. The control group consisted of epithelial cultures carried out on hydrogel with no coating. Cellular migration was quantified, and statistically analyzed. 8-day cultures were processed for transmission electron microscopy.
Results:
Epithelial migration on hydrogel was significantly increased (p < 0.001) in the heparan sulfate proteoglycan group, significantly decreased (p < 0.05 and p < 0.001) with laminin or type IV collagen coating, and showed no significant modifications in the fibronectin group. Transmission electronic microscopy showed a monolayer of epithelial cells adherant to the surface of the hydrogel. Deposit of extracellular matrix and sketch of adhesion focal points were also noted.
Conclusion:
LLH480 cytocompatibility can be improved by heparan sulfate proteoglycan coating.