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[Carboxypeptidases from different microbiolorganisms]

G S Piliavs'ka, O S Tsiperovich

    Ukrains'Kyi Biokhimichnyi Zhurnal
    |November 1, 1977
    PubMed
    Summary
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    This study investigated alkali and acid carboxypeptidases in various microorganisms. Fungi primarily produce acid carboxypeptidases, while actinomycetes yield alkali carboxypeptidases, with Streptomyces griseus showing high activity.

    Area of Science:

    • Biochemistry
    • Microbiology
    • Enzymology

    Background:

    • Carboxypeptidases are crucial enzymes involved in protein degradation.
    • Understanding the distribution and characteristics of alkali and acid carboxypeptidases across different microbial groups is essential for biotechnological applications.

    Purpose of the Study:

    • To detect and characterize alkali and acid carboxypeptidases in a diverse range of microbial sources.
    • To compare the distribution patterns and production levels of these enzymes in fungi, yeast, actinomycetes, bacteria, and algae.

    Main Methods:

    • Enzymic preparations from 39 microbial sources were analyzed for the presence of alkali and acid carboxypeptidases.
    • Comparative analysis of enzyme distribution, activity, and stability was performed.

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    Main Results:

    • Significant variations in carboxypeptidase distribution were observed across microbial taxa.
    • Fungi predominantly synthesize acid carboxypeptidases (e.g., Aspergillus oryzae, Aspergillus flavus), while actinomycetes primarily produce alkali carboxypeptidases (e.g., Streptomyces griseus).
    • Streptomyces griseus exhibited exceptionally high specific activities for its carboxypeptidase preparations, surpassing previously studied enzymes, and demonstrated relative stability during purification.

    Conclusions:

    • Microbial source significantly influences the type and abundance of carboxypeptidases produced.
    • Actinomycetes, particularly Streptomyces griseus, represent a promising source for highly active and stable alkali carboxypeptidases.
    • The distinct enzymatic profiles of fungi and actinomycetes highlight their specialized roles in proteolytic processes.