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[Culturing T-lymphoblastic cell lines in media containing growth proteins]
Voprosy Virusologii
|May 1, 1997
Abstract:
Two optimal variants of growth medium with different content of growth proteins obtained from cattle and porcine blood sera are recommended for culturing lymphoblastoid cells. Use of growth proteins helps optimize the conditions of cell culturing by ruling out the toxic effect and Mycoplasma contamination and ensures the standardization of virological experiments due to the absence of gamma-globulin fraction.