Skeletal and cardiac alpha-actin isoforms exhibit unanticipated temporal and tissue-specific gene expression patterns...

The initial expression of skeletal muscle structural genes typically occurs after myogenic determination factor gene expression. We investigated this temporal relationship via a reverse transcription-polymerase chain reaction (RT-PCR) analysis of skeletal and cardiac alpha-actin (s- and c-actin) mRNA during chick limb development. c-actin transcripts were first detected at the beginning of muscle cytodifferentiation in stage 24/25 limbs, shortly after the initial appearance of MyoD and myogenin mRNAs, and were not detected in nonmyogenic tissues. In contrast, s-actin mRNA was detected in limbs at stage 15-16, periods when myf5 and MRF4 but not MyoD and myogenin transcripts are present (Lin-Jones, J., and Hauschka, S. D., Dev. Biol. 174, 407-422, 1996). While s-actin mRNA was not detected in E7 neural retina and was at variable levels in stage 9-15 neural tube, significant levels were consistently detected in mesodermal tissues which contribute nonmyogenic cells to the limb: stage 9-12 lateral plate and distal portions of stage 25/26 limbs. s-actin transcripts detected in the earliest limbs could thus be in myogenic and/or nonmyogenic cells. These data indicate that while c-actin expression is activated at the onset of limb muscle cytodifferentiation, s-actin expression occurs much earlier, as well as in some nonmyogenic tissues. Whether the precocious expression of s-actin plays a functional role in muscle development remains to be determined.