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Related Experiment Videos

Developmental changes in ob gene expression and circulating leptin peptide concentrations

S U Devaskar1, C Ollesch, R A Rajakumar

  • 1Department of Pediatrics, University of Pittsburgh, Magee-Womens Research Institute, Pennsylvania 15213-3180, USA.

Biochemical and Biophysical Research Communications
|September 23, 1997
PubMed
Summary

Leptin mRNA and peptide levels are elevated in early postnatal mice, particularly during high-fat milk consumption. This suggests temporary hypothalamic leptin receptor resistance in newborns.

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Area of Science:

  • Endocrinology
  • Developmental Biology
  • Metabolism

Background:

  • Leptin regulates energy balance and adiposity.
  • Understanding leptin's developmental trajectory is crucial for metabolic health.
  • Adipose tissue is a primary source of leptin.

Purpose of the Study:

  • To investigate developmental changes in leptin mRNA and peptide levels in murine adipose tissue.
  • To compare leptin expression between white and brown adipose tissue.
  • To examine potential sexual dimorphism in leptin levels during postnatal development.

Main Methods:

  • Quantification of leptin mRNA using approximately 4.4 kb transcript levels.
  • Measurement of circulating immunoreactive total leptin peptide concentrations.

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  • Analysis of adipose tissue from different postnatal ages (2d to 160d) and sexes.

    • Main Results:

    • Leptin mRNA and peptide levels were significantly higher in early postnatal stages (2-14d) compared to adult levels.
    • No significant differences in mRNA levels were found between brown and white adipose tissue.
    • Mildly higher mRNA levels in males were observed at 2 and 7 days, but no sexual dimorphism in peptide levels.
    • Elevated leptin levels correlated with high-fat milk diet and increased body weight gain.

    Conclusions:

    • Leptin expression is developmentally regulated, peaking in the early postnatal period.
    • High postnatal leptin levels during high-fat feeding may indicate transient hypothalamic leptin receptor resistance.
    • These findings contribute to understanding early-life metabolic programming and leptin signaling.