Related Experiment Videos
Primer/template-independent synthesis of poly d(A-T) by Taq polymerase
K Hanaki1, T Odawara, T Muramatsu
1Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.
Biochemical and Biophysical Research Communications
|September 23, 1997
Abstract:
Taq DNA polymerase polymerized dATP and dTTP to poly d(A-T) without requiring added primer/template in the temperature range of 60-70 degrees C. Tth DNA polymerase also catalyzed the reaction, while delta Tth, Vent, Vent(exo-), Pfu, Ultma, BcaBEST, and KOD DNA polymerases did not. The reaction was distinct from the template-nonrequiring terminal deoxynucleotidyl transferase reaction which absolutely required primers.