[Immunohistochemical identification of specific antigens in stained and balsam-embedded eye lens sections]

Localization of specific proteins at preliminary stained with azan and embedded in balsam sections of amphibian eye lens (Rana temporaria, Xenopus laevis) was studied by indirect immunofluorescence. The analysis was performed on the lens of intact animals as well as on lens induced in amphibia gastrula ectoderm in vitro. To remove balsam the lens sections were successively washed by xylene, ethanol and saline (pH 7.1). Then they were treated according to the general principles of immunohistochemical analysis. Specific antigens have been thus shown to be detectable at lens sections which had been kept in balsam for over a year. The immunofluorescent reaction to lens proteins was negative at more prolonged storage of the sections (2--3 years).