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Packed red cell transfusions -- improved survival, quality and storage

V A Lovric, B Prince, J Bryant

    Vox Sanguinis
    |December 1, 1977
    PubMed
    Summary

    Researchers found that citrate phosphate dextrose adenine (CPDA) solution with adenine best preserved red blood cells for 28 days. Rejuvenation further improved cell function and safety for transfusion.

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    Area of Science:

    • Blood banking and transfusion medicine
    • Hematology
    • Biochemistry

    Background:

    • Optimizing red blood cell storage is crucial for transfusion efficacy.
    • Current anticoagulant-preservative solutions have limitations in extending shelf life and maintaining cell viability.
    • Erythrocyte metabolic activity declines during storage, impacting post-transfusion outcomes.

    Purpose of the Study:

    • To evaluate various anticoagulant-preservative solutions for optimal packed red blood cell shelf life.
    • To assess the impact of a rejuvenating solution on stored erythrocyte function and safety.
    • To determine the feasibility of using rejuvenated blood in clinical transfusions.

    Main Methods:

    • Comparison of different anticoagulant-preservative solutions, including citrate phosphate 277 mM dextrose, 0.25 mM adenine (CP2 X D-adenine).
    • Addition of a rejuvenating electrolyte solution using a closed plastic pack system.
    • Assessment of 24-hour post-transfusion survival, erythrocyte viability, oxygen delivery capacity, and metabolic activity.
    • Monitoring of plasma potassium levels and microaggregate formation.
    • Clinical transfusion of rejuvenated blood units with observation for adverse reactions.

    Main Results:

    • CP2 X D-adenine achieved satisfactory 24-hour post-transfusion survival (>70%) after 28-day storage.
    • Rejuvenation significantly improved erythrocyte viability, oxygen delivery, and metabolic activity.
    • Rejuvenation led to reduced plasma potassium levels and fewer microaggregates.
    • Transfusion of 440 units of rejuvenated blood showed no adverse reactions.

    Conclusions:

    • CP2 X D-adenine is an effective solution for extending packed red blood cell shelf life to 28 days.
    • Erythrocyte rejuvenation offers a viable strategy to enhance the quality and safety of stored blood.
    • Rejuvenated blood is safe for clinical transfusion, improving outcomes for recipients.

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