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Determination of naphthodianthrones in plant extracts from Hypericum perforatum L. by liquid
G Piperopoulos1, R Lotz, A Wixforth
1Eberhard-Karls-Universität, Institut für Organische Chemie, Tübingen, Germany.
Abstract:
The determination of the naphthodianthrone constituents in extracts of dried blossoms of Hypericum perforatum L. by combined HPLC-electrospray mass spectrometry is described. Hypericin (1), pseudohypericin (2) and their precursor compounds produce intensive negative quasi-molecular ions by deprotonation provided a non-acidic eluent system is used in the HPLC separation. From the [M-H]- ions formed in the electrospray ionization process characteristic daughter ion spectra can be obtained by collisional activation which have been studied by tandem mass spectrometry.