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Related Experiment Videos

Delay of CNTF decrease following peripheral nerve injury in C57BL/Wld mice

M C Subang1, M A Bisby, P M Richardson

  • 1Division of Neurosurgery, Montreal General Hospital and McGill University, Quebec, Canada.

Journal of Neuroscience Research
|September 25, 1997
PubMed
Summary

Axonal contact delays the reduction of ciliary neurotrophic factor (CNTF) mRNA during nerve injury. In Wld mice, CNTF protein levels remain stable longer than mRNA levels following Wallerian degeneration.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Ciliary neurotrophic factor (CNTF) expression in Schwann cells decreases during Wallerian degeneration.
  • CNTF expression patterns resemble those of myelin protein genes in peripheral nerves.

Purpose of the Study:

  • To investigate the role of axonal contact in regulating CNTF synthesis.
  • To analyze CNTF expression dynamics during Wallerian degeneration in C57BL/Wld mice.

Main Methods:

  • Western blot analysis to assess CNTF protein levels.
  • Reverse transcriptase polymerase chain reaction (RT-PCR) to quantify CNTF mRNA concentrations.
  • Utilizing C57BL/Wld mice with delayed Wallerian degeneration.

Main Results:

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  • CNTF immunoreactivity was significantly higher in Wld nerves compared to control nerves 10 days post-ligation.
  • CNTF mRNA levels decreased less in Wld mice than in control mice at 4 days post-ligation.
  • At 10 days post-ligation, CNTF mRNA levels were comparably low in both Wld and control mice, while protein decrease was delayed in Wld mice.

Conclusions:

  • Maintenance of axonal contact delays the reduction of CNTF mRNA following nerve injury, even without axonal transport.
  • The decrease in CNTF protein during Wallerian degeneration is delayed longer than the decrease in CNTF mRNA in Wld mice.