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Monoclonal antibody E8-18 identifies an integral membrane surface protein unique to Mycoplasma capricolum subsp.
F R Rurangirwa1, P S Shompole, A N Wambugu
1Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA.
Clinical and Diagnostic Laboratory Immunology
|September 25, 1997
Abstract:
Monoclonal antibody (MAb) E8-18 reacted with four isolates of Mycoplasma capricolum subsp. capripneumoniae in Western blots identifying an epitope on a 24 kDa antigen (p24). MAb E8-18 did not react with 11 isolates belonging to four other Mycoplasma species or subspecies closely related to M. capricolum subsp. capripneumoniae. A combination of trypsin treatment of intact organisms and detergent-phase partitioning revealed p24 to be an integral M. capricolum subsp. capripneumoniae surface membrane protein.