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Detection of rotavirus in sewage and creek water: efficiency of the concentration method
D U Mehnert1, K E Stewien, C M Hársi
1Departamento de Microbiologia, Instituto de Cièncias, Biomédicas, Universidade de São Paulo, Brasil.
Memorias Do Instituto Oswaldo Cruz
|January 1, 1997
Abstract:
Simian rotavirus SA-11 experimentally seeded, was recovered from raw domestic sewage by a two-step concentration procedure, using filtration through a positively charged microporous filter (Zeta Plus 60 S) followed by ultracentrifugation, effecting an 8,000-fold concentration. By this method, a mean recovery of 81% +/- 7.5 of the SA-11 virus, was achieved.