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Methods for detecting apoptotic cells in tissues

E J Sanders1

  • 1Department of Physiology, University of Alberta, Edmonton, Canada.

Histology and Histopathology
|September 26, 1997
PubMed
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This review surveys methods for detecting cell death in tissues, focusing on in situ apoptosis recognition. It evaluates techniques for identifying apoptosis versus necrosis at various stages.

Area of Science:

  • Cell Biology
  • Histopathology

Background:

  • Accurate detection of cell death is crucial in biological research and disease diagnosis.
  • Distinguishing between apoptosis and necrosis is essential for understanding cellular processes.

Purpose of the Study:

  • To review and compare current methods for detecting cell death in tissues.
  • To highlight techniques for in situ identification of apoptotic cells.
  • To assess the utility of methods in differentiating apoptosis from necrosis.

Main Methods:

  • Survey of in situ DNA nick-end labeling techniques.
  • Evaluation of vital dyes for cell death detection.
  • Analysis of lysosomal enzyme histochemistry.
  • Assessment of transglutaminase expression.

Related Experiment Videos

  • Review of immunocytochemical detection of death-associated antigens.

    • Main Results:

    • Various methods allow in situ recognition of apoptotic cells in whole mounts and tissue sections.
    • Techniques differ in their ability to detect different stages of cell death.
    • The capacity to distinguish between apoptotic and necrotic cell death varies among methods.

    Conclusions:

    • A range of techniques are available for cell death detection in tissues.
    • Selection of method depends on the specific research question and desired information.
    • Further refinement of methods can improve discrimination between apoptotic and necrotic cell death.