Characterization of microsomal GST-II by western blot and identification of a novel LTC4 isomer

¹Merck Frosst Centre For Therapeutic Research, Kirkland, Quebec, Canada. Per-Johan.Jakobsson@mbb.ki.se

Journal of Lipid Mediators and Cell Signalling

|September 26, 1997

Abstract:

Protein expression of microsomal GST-II and LTC4 synthase was analyzed by Western blot. Correlation between a 17 kDa band and LTC4 formation was observed for both enzymes. The expression of microsomal GST-II was several fold more efficient than the expression of LTC4 synthase. In addition to catalyzing the biosynthesis of LTC4, microsomal GST-II also produces another product, which has been subjected to mass spectrometric analysis. This analysis demonstrates that the novel product is an isomer of LTC4.

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Abstract:

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