Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bioavailability Enhancement: Drug Solubility Enhancement

Bioavailability Enhancement: Drug Solubility Enhancement

Body:Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...

Bioavailability Enhancement: Drug Permeability Enhancement

Bioavailability Enhancement: Drug Permeability Enhancement

Body:After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt...

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

Body:Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...

Self-Evaluation: Self-Enhancement and Self-Verification

Self-Evaluation: Self-Enhancement and Self-Verification

Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...

Cancer Stem Cells and Tumor Maintenance

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...

Enhanced Elimination of Poison

Enhanced Elimination of Poison

Poison can be effectively removed from the gastrointestinal (GI) tract through various decontamination procedures.
Antidotes serve a crucial role in counteracting the effects of poison by inhibiting enzymes responsible for producing harmful drug metabolites. In some cases, these toxic metabolites can be neutralized by endogenous cosubstrates, which are maintained at specific concentrations to prevent interaction with cellular macromolecules and subsequent cell death.
Renal excretion is the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Stereotactic radiosurgery with immunotherapy is associated with improved overall survival in patients with metastatic melanoma or non-small cell lung cancer: a National Cancer Database analysis.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2021

Same author

Localized synchronous squamous cell carcinomas of the esophagus and hypopharynx treated with definitive concurrent chemoradiotherapy with a unified radiotherapy plan.

Current oncology (Toronto, Ont.)·2014

Same author

CT-Guidance Allows Interstitial Implantation in an Outpatient Setting for Cervical Cancer Patients.

Practical radiation oncology·2014

Same author

Use of Implanted Gold Fiducial Markers With MV-CBCT Image Guided IMRT for Pancreatic Tumors.

Practical radiation oncology·2014

Same author

Pretreatment SUVmax as a Marker for Progression-Free Survival in Stage I NSCLC Treated With SBRT.

Practical radiation oncology·2014

Same author

Re-irradiation with radiosurgery for recurrent glioblastoma multiforme.

Technology in cancer research & treatment·2011

Search research articles

Related Experiment Video

Updated: Feb 6, 2026

Author Spotlight: Radiotherapy and Clonogenic Assays for Advancing Cancer Research and Personalized Medicine

Author Spotlight: Radiotherapy and Clonogenic Assays for Advancing Cancer Research and Personalized Medicine

Published on: April 5, 2024

Hypothermia-enhanced human tumor cell radiosensitivity

S A Burton¹, W R Paljug, S Kalnicki

¹Department of Radiation Oncology, Allegheny University of the Health Sciences, Pittsburgh, Pennsylvania 15212-9986, USA.

|August 1, 1997

Summary

Hypothermia, or cooling, enhances cancer cell radiosensitivity. This effect is dependent on cooling duration and temperature, with rapid decay upon rewarming, suggesting impaired sublethal damage repair.

More Related Videos

Enhancing Tumor Content through Tumor Macrodissection

Enhancing Tumor Content through Tumor Macrodissection

Published on: February 12, 2022

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model

Published on: October 27, 2020

Related Experiment Videos

Last Updated: Feb 6, 2026

Author Spotlight: Radiotherapy and Clonogenic Assays for Advancing Cancer Research and Personalized Medicine

Author Spotlight: Radiotherapy and Clonogenic Assays for Advancing Cancer Research and Personalized Medicine

Published on: April 5, 2024

Enhancing Tumor Content through Tumor Macrodissection

Enhancing Tumor Content through Tumor Macrodissection

Published on: February 12, 2022

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model

Published on: October 27, 2020

Area of Science:

Cryobiology
Radiation Oncology
Cancer Cell Biology

Background:

Cryoablation is an emerging therapeutic approach for neoplastic diseases.
Understanding the impact of cooling on cellular radiation response is crucial for optimizing cancer therapies.

Purpose of the Study:

To investigate the influence of hypothermic conditions on the radiosensitivity of human cervical carcinoma cells (HTB35).
To determine the effects of varying cooling temperatures, durations, and rewarming intervals on cellular survival post-irradiation.

Main Methods:

Human cervical carcinoma cells (HTB35) were exposed to temperatures of 0, 5, or 15 degrees C for up to 24 hours.
Cells were irradiated post-cooling, and survival was assessed using in vitro clonogenic assays.
Flow cytometry was employed to analyze cell cycle distribution (S-phase fraction).

Main Results:

Cooling for 24 hours significantly reduced cell survival to 75% of control.
X-irradiation after 24-hour cooling resulted in a 1.6-fold increase in radiosensitivity.
The radiosensitizing effect diminished rapidly if cells were rewarmed for 6 hours or more before irradiation.

Conclusions:

Cooling enhances in vitro radiation sensitivity in a manner dependent on temperature, duration, and rewarming interval.
Cell cycle redistribution does not appear to mediate this enhanced radiosensitivity.
Impaired sublethal damage repair capacity may significantly contribute to the observed increase in radiosensitivity following cooling.