Atomic force microscopy as a tool for study of human hair

Area of Science:

• Materials Science
• Biophysics
• Surface Science

Background:

• Atomic force microscopy (AFM) provides high-resolution, non-invasive 3D imaging of biological materials.
• Understanding human hair structure is crucial for cosmetic and dermatological applications.
• Conventional microscopy techniques have limitations in analyzing biological samples in their native state.

Purpose of the Study:

• To investigate the effects of varying pH buffer solutions and heat on human hair structure using AFM.
• To assess the quantitative changes in hair cuticle height as an indicator of structural alterations.
• To evaluate AFM as a viable alternative to traditional microscopy for hair research.

Main Methods:

• Human hair samples were subjected to treatments with phosphate-buffered saline at pH 3.0, 7.0, and 11.0.
• Hair samples were also exposed to controlled heating for varying durations.
• Atomic force microscopy was employed for high-resolution imaging and quantitative analysis of hair surface topography, focusing on cuticle height.

Main Results:

• Hair cuticle height significantly changed in response to pH and heat treatments.
• Hair swelling decreased as pH increased, indicating reduced water uptake at higher pH.
• Heating induced both expansion and shrinkage of hair structure, dependent on treatment duration.

Conclusions:

• The hair cuticle serves as a sensitive marker for evaluating structural damage or changes induced by external factors.
• AFM is a powerful tool for quantitative assessment of hair structure modifications.
• AFM offers a valuable alternative to conventional microscopy for detailed hair research.