[Simplified gas chromatography determination of free and total cholesterol]

Gas-liquid chromatographic methods are more exact than colorimetric and enzymatic procedures for the estimation of cholesterol. In this paper an exact and very quick method of determination of cholesterol is described. This is attainable by omittance of any extraction and by addition of stigmasterol as an internal standard and simultaneous denaturation of the proteins. The method is well suitable for determination of the activity of lecithin-cholesterol acyltransferase, a determination which requires not only accuracy, but also the exact moment of the enzymatic inactivation.