Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Temperature-controlled high-performance liquid chromatography using a uniformly sized temperature-responsive

K Hosoya1, K Kimata, T Araki

  • 1Department of Polymer Science, Kyoto Institute of Technology, Japan.

Analytical Chemistry
|June 1, 1995
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tensioning diaphragmoplasty for treating bilateral phrenic nerve paralysis in a dog.

The Journal of small animal practice·2025
Same author

Urinary steroid profiling using liquid chromatography-tandem mass spectrometry for the diagnosis of canine Cushing's syndrome.

Veterinary journal (London, England : 1997)·2024
Same author

Direct renal pelvicocystostomy using tube cystoplasty in a cat with ureteral obstruction.

The Journal of small animal practice·2022
Same author

Epidemiology of massive hepatocellular carcinoma in dogs: A 4-year retrospective study.

Veterinary journal (London, England : 1997)·2019
Same author

Impact of surgical margins on survival of 37 dogs with massive hepatocellular carcinoma.

New Zealand veterinary journal·2017
Same author

Comparative analysis of mRNA expression of surface antigens between histiocytic and nonhistiocytic sarcoma in dogs.

Journal of veterinary internal medicine·2014

Researchers developed novel polymer-based packing materials for chromatography. These materials, modified with temperature-responsive polymers, offer thermally controlled separation selectivity for drugs and improved analysis of proteins like BSA.

Area of Science:

  • Chromatography
  • Polymer Science
  • Materials Science

Background:

  • Chromatographic separation relies on stationary phase properties.
  • Temperature-responsive polymers offer tunable material characteristics.
  • Controlling selectivity in reversed-phase high-performance liquid chromatography (RP-HPLC) is crucial for complex separations.

Purpose of the Study:

  • To synthesize and characterize polymer-based packing materials with temperature-responsive selectors.
  • To evaluate the thermally controlled separation selectivity of these materials in RP-HPLC.
  • To explore applications in drug separation and direct serum sample analysis.

Main Methods:

  • In situ surface-selective modification of polymer-based packing materials.
  • Incorporation of poly(N-isopropylacrylamide) as a temperature-responsive selector.

Related Experiment Videos

  • Utilizing modified materials as stationary phases in reversed-phase high-performance liquid chromatography.

    • Main Results:

    • Packing materials with internal surface modification demonstrated temperature-dependent separation selectivity for drugs.
    • Improved drug separation resolution was achieved above the phase transition temperature of the selector.
    • Externally modified materials showed temperature-responsive elution shifts for polypeptides (e.g., BSA), enabling potential direct serum injection.

    Conclusions:

    • Surface-modified polymer-based packing materials offer effective thermal control over chromatographic selectivity.
    • These materials show promise for enhanced drug separations and simplified protein analysis.
    • The developed stationary phases represent a novel approach to tunable chromatographic separations.