Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[NF1 (neurofibromatosis type 1)]

H Maruta1, Nur-e-Kamal

  • 1Ludwig Institute for Cancer Research, Melbourne.

Gan to Kagaku Ryoho. Cancer & Chemotherapy
|October 6, 1997
PubMed
Summary

Ras GTPase activating proteins (GAPs) like NF1 regulate normal Ras activity. NF1 fragments and Raf81 compete with oncogenic Ras for binding, suppressing tumor growth in Ras-associated cancers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PAK1-deficiency/down-regulation reduces brood size, activates HSP16.2 gene and extends lifespan in Caenorhabditis elegans.

Drug discoveries & therapeutics·2013
Same author

From chemotherapy to signal therapy (1909-2009): A century pioneered by Paul Ehrlich.

Drug discoveries & therapeutics·2012
Same author

Ivermectin inactivates the kinase PAK1 and blocks the PAK1-dependent growth of human ovarian cancer and NF2 tumor cell lines.

Drug discoveries & therapeutics·2012
Same author

The direct PAK1 inhibitor, TAT-PAK18, blocks preferentially the growth of human ovarian cancer cell lines in which PAK1 is abnormally activated by autophosphorylation at Thr 423.

Drug discoveries & therapeutics·2012
Same author

Effective neurofibromatosis therapeutics blocking the oncogenic kinase PAK1.

Drug discoveries & therapeutics·2012
Same author

CAPE (caffeic acid phenethyl ester)-based propolis extract (Bio 30) suppresses the growth of human neurofibromatosis (NF) tumor xenografts in mice.

Phytotherapy research : PTR·2008

Area of Science:

  • Molecular biology
  • Oncology
  • Biochemistry

Context:

  • Ras GTPase activating proteins (GAPs) regulate Ras signaling by stimulating GTPase activity.
  • Normal Ras is GDP-bound (inactive), while oncogenic Ras is GTP-bound (active).
  • NF1 is a tumor suppressor linked to neurofibromatosis type 1, caused by elevated active Ras (T-Ras).

Purpose:

  • To identify minimum Ras-binding domains within NF1 and Raf.
  • To investigate the competitive binding of NF1 and Raf fragments to T-Ras.
  • To evaluate the potential of these fragments in suppressing oncogenic Ras-driven tumors.

Summary:

  • NF1 fragments (NF78, NF56) and a Raf fragment (Raf81) were identified as minimum Ras-binding domains.
  • These NF1 and Raf fragments compete with oncogenic Ras (T-Ras) for binding to effector domain I.
  • Overexpression of NF1 or Raf fragments suppressed malignant transformation induced by oncogenic Ras mutants.

Impact:

  • Identified key domains for Ras binding and regulation.
  • Demonstrated competitive inhibition of oncogenic Ras signaling.
  • These findings offer therapeutic strategies for T-Ras-associated tumors, including neurofibromatosis type 1.
  • Potential for controlling proliferation in over 30% of human carcinomas.

Related Experiment Videos