[Phenotype and genotype analysis of Torulopsis glabrata isolated from nosocomial infections]

Twenty-one strains of Torulopsis glabrata have been isolated in the hospital of Versailles during a three months period, in eight patients, and related to nosocomial infections. In order to know if they have been cross infected, a genotypic analysis (pulsed field electrophoresis) has been performed. The strains from four patients had the same karyotype, suggesting they had probably crossed infections. Since this kind of analysis is expensive and time consuming, we have tried a more practical approach. The susceptibility of the strains has been tested by Etest for five antifungal agents: amphotericin B, 5-fluorocytosine, ketoconazole, fluconazole et itraconazole. Statistical analysis of these results has not given the same classification of the strains of T. glabrata. Karyotyping by pulsed field electrophoresis of strains seems to be a better method to confirm strain clonality.