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Related Experiment Videos

Nuclear antigen expressed by proliferating cells

A Peled1, E Shezen, D Schwartz

  • 1Department of Neurology, Hadassah-Hebrew University Hospital, Jerusalem, Israel.

Hybridoma
|August 1, 1997
PubMed
Summary
This summary is machine-generated.

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A new monoclonal antibody, PRA-72, identifies a nuclear antigen linked to cell proliferation. This antibody shows promise for evaluating human tumors by detecting proliferating cells.

Area of Science:

  • Immunology
  • Cell Biology
  • Oncology

Background:

  • Cell proliferation is a fundamental process in growth and development.
  • Identifying markers of cell proliferation is crucial for understanding cancer and developing therapies.

Purpose of the Study:

  • To characterize a novel monoclonal antibody (PRA-72) that targets a nuclear antigen associated with cell proliferation.
  • To evaluate the potential utility of PRA-72 in identifying proliferating cells and its application in tumor evaluation.

Main Methods:

  • Production and characterization of a novel mouse monoclonal antibody (PRA-72).
  • Western blot analysis to identify the target nuclear protein.
  • Immunocytological studies on cells in different cell cycle phases.
  • Immunohistochemical analysis of various mouse and human tissues.

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Main Results:

  • PRA-72 specifically stains the nuclei of proliferating stromal cells, with staining disappearing in quiescent (Gzero) cells.
  • Western blot identified a 35-40 KD nuclear protein doublet, present in proliferating but not confluent cells.
  • The antigen is peripherally located in the nucleus except during mitosis, where it envelops chromosomes.
  • PRA-72 selectively stained proliferating hemopoietic precursors in co-cultures and demonstrated differential expression in various tissues, including human colon carcinoma.

Conclusions:

  • The novel monoclonal antibody PRA-72 recognizes a nuclear antigen selectively expressed by proliferating cells.
  • PRA-72 shows potential as a diagnostic tool for evaluating cell proliferation in various tissues, including human tumors.